Legacy methods developed during the early days of HPLC technology have not kept pace with recent advancements and modernizations in the field. Advancements in column chemistry and system robustness ...

Tacrolimus is an immunosuppressant macrolactam of fermentative origin. By means of HPLC, LC-MS and NMR analyses, coupled with the reference standard synthesis, the main impurities of tacrolimus bulk ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

In this module, first the types of LC system classifications are explained. Following this, the analytical columns used in liquid chromatography are covered in detail (stationary phase, particle size ...

Supercritical fluid chromatography, or SFC, has been around for decades. During that time, the separation technique has fallen in and out of and back in favor. But it has consistently been relegated ...