A wave of spatial transcriptomics studies has produced gene-expression atlases that span entire organs and whole organisms, from mouse embryos to the roundworm C. elegans to 31 human tissues. These ...

Tumors contain many different types of cells organized in complex spatial patterns that can influence how the disease progresses. Because of this, it is hard to predict how a tumor will develop and ...

Spatial transcriptomics is a technique that provides information about gene expression patterns within intact tissues. This technology employs various methodologies, including in situ sequencing (ISS) ...

This Research Topic is the second volume of the “Unraveling Breast Cancer Complexity: Insights from Single-Cell Sequencing and Spatial Transcriptomics” ...

Biological systems are inherently three-dimensional—tissues form intricate layers, networks, and architectures where cells interact in ways that extend far beyond a flat plane. To capture the true ...

Conventional transcriptomic techniques have revealed much about gene expression at the population and single-cell level—but they overlook one crucial factor: spatial context. In musculoskeletal ...

Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the ...