SOLVED: Calculate the number of uL of each lysate to add to each of the samples to get 25ug of total protein. Include the equation from your standard curve trendline. The maximum amount you can load in your wells is approximately 16uL, where 4uL is the loading buffer. Therefore, your sample cannot exceed 12uL. If your lysate isn't concentrated enough to get 25ug in less than 12uL, you will need to lower the amount of protein added for all the samples to the amount of protein that can be loaded f
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